WP2: Passive case detection

Work Package 2 Overview

Goal:

To determine the diagnostic performance and cost-effectiveness of rapid diagnostic tests (RDTs) in peripheral health centres in low prevalence HAT foci and to establish diagnostic algorithms combining RDTs at point-of-care, and remote serological and/or molecular reference tests on blood impregnated filter paper.

This work package deals with passive case detection, as increased integration of case detection is the way to go in HAT control. We aim at establishing improved test algorithms, enabling health centres to select for those clinical suspects and RDT positives that need further attention and confirmatory parasitological analyses (or on the long term maybe treatment). We will perform all available rapid diagnostic tests in parallel on clinical suspects presenting in peripheral health centres. All individuals positive in at least one RDT, will undergo sensitive parasitological confirmation. Blood on filter paper is taken for remote testing in a reference centre using ELISA, trypanolysis, Real time-PCR and LAMP (WP5). The results from reference analysis will be used to decide on parasitological follow-up examinations: Reference test positives not yet parasitologically confirmed will be revisited for parasitological examination.

Countries involved into Work package 2 are DR Congo, Guinea, and Côte d’Ivoire with respectively PNLTHA-DRC, PNLTHA-GN and IPR being the main participants involved.

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